Isolation of natural inhibitors of papain obtained from Carica papaya latex
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چکیده
منابع مشابه
Isolation of Natural Inhibitors of Papain Obtained from Carica papaya Latex
Studies were carried out to natural papain inhibitor from papaya latex. Fresh latex from green fruits of Carica papaya was collected and immediately transported in ice bath to the lab, from which three fractions with inhibitor effect of esterase papain activity were isolated by latex dialysis, Sephadex G-25 gel filtration and ionic exchange chromatography in SP-Sephadex C-25. The isolated fract...
متن کاملIn vitro efficacy of latex and purified papain from Carica papaya against Strongyloides venezuelensis eggs and larvae
Latex from Carica papaya is rich in bioactive compounds, especially papain, which may help to control parasitic diseases. This study evaluated the efficacy of latex from C. papaya and purified papain against Strongyloides venezuelensis. The Egg Hatching Test (EHT) and the Larval Motility Test (LMT) using fresh and frozen latex (250mg/mL), lyophilized latex (34mg/mL), and purified papain (2.8 mg...
متن کاملAffinity purification of the novel cysteine proteinase papaya proteinase IV, and papain from papaya latex.
A procedure is described for the purification of a previously undetected cysteine proteinase, which we have called papaya proteinase IV, from spray-dried latex of the papaya (Carica papaya) plant. The purification involves affinity chromatography on Gly-Phe-aminoacetonitrile linked to CH-Sepharose 4B, with elution by 2-hydroxyethyl disulphide at pH 4.5. The product thus obtained is a mixture of...
متن کاملاستخراج، شناسایی و بررسی فعالیت آنزیم پاپائین میوه Carica papaya از ایران
Background and purpose: Papain, a proteolytic enzyme, is found in the dried and purified latex from the fruits of Carica papaya L. (Caricaceae). Papain is used in medicine as a digestive and in the debridement of necrotic tissues. It is used in pharmaceutics as a spreading agent for drugs, supplied as an ointment (10%) with urea which denatures nonviable protein matter present in lesions. ...
متن کاملA two-step procedure for purification of papain from extract of papaya latex.
A method is presented for purifying papain from extracts of papaya latex. The procedure involves precipitation of the extract of papaya latex with sodium chloride followed by affinity chromatography of the redissolved precipitate. Precipitation of the protein from the latex extract is necessary to separate the papain from material which interferes with the binding of papain to the affinity colu...
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ژورنال
عنوان ژورنال: Brazilian Archives of Biology and Technology
سال: 2004
ISSN: 1516-8913
DOI: 10.1590/s1516-89132004000500010